Cresyl Violet acetate is a red fluorescent stain, which can be used to stain neurons. In Vitro: The estimated total number of SG neurons is 27,485±3251 and 26,705±1823 in the PV and Cresyl Violet acetate stained sections, respectively. There is no significant difference between them (p=0.552). Therefore, Cresyl Violet acetate staining is simpler and more cost effective when estimates neuronal number. Although PV stains spiral ganglion neurons (SGNs) with a greater intensity and provides a functional status, its tedious protocol limits its use for quantification. Total RC volume is estimated using probe and it is found that an average RC volume of 2.162±0.35 mm³ and 1.82±0.33 mm³ in Cresyl Violet acetate staining and PV immunostaining sections, respectively. Volume of neurons is estimated using nucleator probe and it is 3487.63±951 μm³ and 3740.1±784 μm³ in Cresyl Violet acetate staining and PV immunostaining sections, respectively. Similarly, volume of neuronal nucleus is also estimated using nucleator probe and it is found to be 131.68±50 μm³ and 126.51±33 μm³ in Cresyl Violet acetate staining and PV immunostaining sections, respectively^[1].