EB-47 is a potent and selective PARP-1/ARTD-1 inhibitor with an IC₅₀ value of 45 nM, shows modest potency against ARTD5 with an IC₅₀ value of 410 nM. EB-47 mimics the substrate NAD⁺ and extends from the nicotinamide to the adenosine subsite^[1]. In Vitro: EB-47 shows inhibition in excess of 50% with CdPARP, and it is able to inhibit CdPARP and HsPARP with IC₅₀ values of 0.86 and 1.0 µM, respectively^[1].
In Vivo: EB-47 (2 μM; 5 days) decreases the number of embryo implantation sites and blastocysts at day 5. PARP1 participates in the process of embryo implantation^[3].