Myriocin, a fungal metabolite isolated from Myriococcum albomyces, Isaria sinclairi and Mycelia sterilia, is a potent inhibitor of serine-palmitoyl-transferase (SPT) and a key enzyme in de novo synthesis of sphingolipids^[1]. Myriocin strongly suppresses replication of both the subgenomic HCV-1b replicon and the JFH-1 strain of genotype 2a infectious HCV^[2], with an IC₅₀ of 3.5 µg/mL for inhibiting HCV infection^[3]. IC50 & Target: Serine-palmitoyl-transferase (SPT)^[1]