Chemical Name |
JC-1 |
CAS Number |
3520-43-2 |
MDL Number |
MFCD00188475 |
Molecular Formula |
C25H27Cl4IN4 |
Molecular Weight |
652.23 |
Synonyms |
CBIC2 |
Introduction of 3520-43-2 :
JC-1 is a fluorescent lipophilic carbocyanine dye used to measure mitochondrial membrane potential. In Vitro: JC-1 (2.5 μM) exposed to murine L1210 lymphoblasts, can be detected the presence of both cytoplasmic JC-1 monomer and mitochondrial J-aggregates in these cells. JC-1 fluorescence is usually excited by the 488 nm laser wavelength common in flow cytometers[1]. Fluorescent labeling of mitochondria with either JC-1 (1 μg/mL, 15 min), reveals that are distributed irregularly, resulting in regions of high and low mitochondrial content within astrocytes[2]. JC-1 has been shown to interact with α-synuclein at the acidic C-terminal region with a Kd of 2.6 μM. JC-1 itself does not accelerate the protein aggregation of α-synuclein in the absence of iron, insted, it decelerates the aggregation process by extending the lag phase approx[3]. JC-1 is avidly accumulated in sensitive K562 cells where it displays both a green cytoplasmic and red mitochondrial fluorescence. JC-1 is poorly accumulated in resistant K562 cells, which displays only a slight green fluorescence[4].
Purity |
NLT 98% |
Storage |
at 20ºC 2 years |
*The above information is for reference only.