CUR61414 is a novel, potent and cell permeable Hedgehog signaling pathway inhibitor (IC₅₀ =100-200 nM). CUR61414 is a small-molecule aminoproline class compound and selectively binds to smoothened (Smo) with a K_i  value of 44 nM. CUR-61414 can induce apoptosis in cancer cells without affecting neighboring non-tumor cells^[1][2]. IC50 & Target: Hedgehog inhibitor^[1] In Vitro: CUR61414 is able to arrest proliferation of basal cells within the BCC-like lesions and induce cells to undergo apoptosis resulting in complete regression of the lesions, without affecting neighboring skin cells^[1].
In Vivo: CUR61414 cause regression of these lesions in the mice, which are exposed to UV light irradiation for 6-9 months, producing many microscopic BCC-like basaloid lesions throughout their skin. Moreover,a significant increase in apoptotic nuclei can be seen in basaloid nests after CUR61414 treatment and no overt toxicity is observed in the skin surrounding the lesions^[1].