Brilliant black BN (E151) is an azo dye and a food colorant^[1]. Brilliant black BN is a promising antiviral agent against EV71 infection via inhibiting the interaction between EV71 and its cellular uncoating factor cyclophilin A. Brilliant black BN has the potential for the investigation of contagious disease^[2]. IC50 & Target: IC50: 2.39 µM- 28.12 µM (various strains of EV71)^[2] In Vitro: Brilliant black BN (E151) inhibits the interaction between EV71 and its cellular uncoating factor cyclophilin A ^[1].
Brilliant black BN (E151) inhibited the infection EV71 -GFP at a concentration of 300 µM as evidenced from the reduced GFP signals. Brilliant black BN (E151)exhibits the inhibition of EV71 -GFP as a dose-dependent manner in infected RD cells, exhibits an IC₅₀ value of 10.1 µM^[1].
Brilliant black BN (E151) is able to inhibit all tested 28 EV71, CVA16 and CVA6 strains. In rhabdomyosarcoma cells, 50% inhibitory concentration 29 of the dye E151 for various strains of EV71 ranged from 2.39 µM to 28.12 µM ,whereas its 50% cytotoxic concentration is 1870 µM ^[1].
In Vivo: Brilliant black BN (E151)(intraperitoneal injection; 200 mg/kg; 14 days)exhibits mild illness with clinical scores less than 3 and subsequently recovered when compared with PBS-group.All E151 270 treated mice are completely protected throughout the experiment^[1].