Protease-Activated Receptor-2 Activating Peptide is an agonist of Protease-Activated Receptor-2 (PAR-2). IC50 & Target: PAR-2^[1] In Vitro: Protease-Activated Receptor-2 Activating Peptide (SLIGRL-NH₂) is an agonist of PAR-2 and MrgprC11^[1]. Protease-Activated Receptor-2 Activating Peptide (SLIGRL-NH₂) causes an L-NAME-inhibited relaxation. Based on SLIGRL-NH₂ causing a concentration-dependent relaxation with an EC₅₀ of 10 µM in endothelium-free preparations in the presence of perivascular adipose tissue (PVAT) , 20 µM is used as a suitable ‘test’ concentration of peptide in subsequent experiments designed to evaluate the effects of potential inhibitors of ADRF release/action. In the endothelium-free aorta preparations, SLIGRL-NH₂ causes a concentration-dependent relaxation in preparations only in the presence of PVAT [+PVAT, -ENDO (endothelium)]^[2].