ID-8 is a DYRK inhibitor, and sustains embryonic stem cell self-renewal in long-term culture. IC50 Value: Target: DYRK in vitro: the combination of Wnt and ID-8 enhanced hESC replating efficiency, and colonies expressed ALP and displayed undifferentiated morphology. members of the DYRK family are direct targets of ID-8 and that ID-8 enhances Wnt-mediated hESC survival and proliferation via inhibition of DYRKs [1]. ID-8 stimulated proliferation at a steady rate similar to LIF. ID-8 is required to maintain nanog, Sox2, and Rex-1 gene expression [2]. In vivo: