2-Iminobiotin (Guanidinobiotin) is a biotin (vitamin H or B7) analog. 2-Iminobiotin is a reversible nitric oxide synthases inhibitor with K_is of 21.8 and 37.5μM for murine iNOS and rat n-cNOS, respectively^[1]. 2-Iminobiotin superimposes on hypothermia protects human neuronal cells from hypoxia-induced cell damage^[2]. IC50 & Target: NO synthases^[1] In Vitro: Application of low concentrations of 2-Iminobiotin (2-IB; 10 ng/mL and 30 ng/mL) superimposed on hypothermia abrogates the hypoxia-induced lactate dehydrogenase (LDH) increase, resulting in LDH levels that were not different from the normoxia control^[1].