2-Methylcitric acid trisodium (Methylcitric acid trisodium) is an endogenous metabolite in the 2-methylcitric acid cycle. 2-Methylcitric acid trisodium accumulates in methylmalonic and propionic acidemias and acts as a marker metabolite. 2-Methylcitric acid trisodium markedly inhibits ADP-stimulated and uncoupled respiration in mitochondria supported by glutamate^[1]. In Vitro: 2-Methylcitric acid trisodium impairs glutamate metabolism and induces permeability transition in brain mitochondria^[1].
2-Methylcitric acid trisodium inhibits glutamate dehydrogenase (GDH) activity at concentrations as low as 0.5 mM^[1].
2-Methylcitric acid trisodium (1 mM, 3 mM; 150 seconds) provokes a significant decrease in mitochondrial membrane potential and induced swelling in Ca²⁺-loaded mitochondria supported by different substrates^[1].
2-Methylcitric acid trisodium behaves as a potent inhibitor of glutamate oxidation by inhibiting glutamate dehydrogenase activity and as a permeability transition inducer, disturbing mitochondrial energy homeostasis^[1].
2-Methylcitric acid trisodium does not significantly impair mitochondrial glutamate transport^[1].
2-Methylcitric acid trisodium does not significantly impair glutamate mitochondrial transport in liver mitochondria^[1].