IM-12 is an inhibitor of GSK-3β, with an IC₅₀ of 53 nM, and also enhances Wnt signalling. IC50 & Target: IC50: 53 nM (GSK-3β)^[1] In Vitro: IM-12 inhibits GSK-3β in ReNcell VM cells, with I₅₀ of 3.8 μM. IM-12 (3 μM) enhances the β-catenin amount, with no further effect at lower or higher concentration. IM-12 (3 μM) also attenuates the proliferation of ReNCell VM cells. IM-12 increases TCF-activity of ReNcell VM^[1].