ML346 is an activator of heat shock protein 70 (Hsp70), with an EC₅₀ of 4600 nM in HeLa cells. IC50 & Target: EC50: 4600 nM (Hsp70, in HeLa cells)^[1] In Vitro: ML346 is an activator of Hsp70, with an EC₅₀ of 4600 nM in HeLa cells. ML346 (10 μM) restores proteostasis, restores CFTR-mediated iodide conductance, and enhances the correct folding of proteins expressed in two different cellular compartments^[1]. ML346 (Compound F1) induces multiple responses and strongly induces Hsp70, the oxidative stress response genes (HO1 and GCLM), and a 2.5-fold upregulation of BiP in WT MEF cells. ML346 (0.5-25 μM) exhibits cytoprotective effects in cells after a 35 min severe heat shock, and also causes a two-fold protection from H₂O₂-induced apoptosis^[2].